PATHOLOGY LABORATORY TECHNIQUES
Associate	TR-NQF-HE: Level 5	QF-EHEA: Short Cycle	EQF-LLL: Level 5

General Information about the Course

Course Code:

PLT201

Course Title:

Pathology Laboratory Techniques Vocational Training-I (IME)

Course Semester:

3. Semester / Fall

Course Credits:

Theoretical	Practical	Credit	ECTS
0	6	3	7

Language of instruction:

Prerequisite of the course:

Type of course:

Necessary

Level of course:

Associate

TR-NQF-HE:5. Master`s Degree

QF-EHEA:Short Cycle

EQF-LLL:5. Master`s Degree

Course Lecturer(s):

Öğr. Gör. Kubilay GÖÇÜCÜ

Purpose and content of the course

Course Objectives:	To use professional knowledge and skills in routine work by working actively in the Pathology Laboratory for 15 working days.
Course Objective:	During the application, students during working hours; routine histopathological, cytopathological procedures and slide work alternately in the block archive.
Mode of Delivery:	Face to face

Learning Outcomes



Knowledge *(Described as Theoritical and/or Factual Knowledge.)*
Skills *(Describe as Cognitive and/or Practical Skills.)*
1) It is included in routine histopathological study (macroscopy, tissue follow-up, embedding, sectioning, HE staining, closure).
2) Prepares, dyes and seals preparations from gynecological and non-gynecological cytological material.
3) Follows conventional histochemistry studies and works with the technician.
4) Follows immunohistochemistry studies and works with the technician.
5) It accompanies the frozen section.
6) Makes slide and block archives.
Competences *(Described as "Ability of the learner to apply knowledge and skills autonomously with responsibility", "Learning to learn"," Communication and social" and "Field specific" competences.)*

Course Topics

Week

Subject

Application of routine histopathological techniques, cytopreparation and application of cytological dyes, working with and/or observing the technician in the application of cryostat, conventional histochemistry and immunohistochemistry techniques.

10)

11)

12)

13)

14)

References:

Uygulama yapılan laboratuvarlara ait çalışma prosedür ve talimatları

Ders - Program Öğrenme Kazanım İlişkisi

No Effect	1 Lowest	2 Average	3 Highest

Ders Öğrenme Kazanımları	1	2	3	4	6
Program Outcomes
1) Have knowledge about Chemistry, Organic Chemistry and Biology.
2) Learns about the human body and its functioning to support their professional practice.	2	1
3) Knows basic macroscopic sampling techniques.	3
4) Knows basic and advanced pathology laboratory diagnostic techniques.	3		2
5) Have knowledge of the necessary terminology in order to follow the current developments in pathology techniques.	3
6) Gains knowledge about the effective use of devices and equipment used in the pathology laboratory.	3
7) Knows the quality standards and safety procedures of pathology laboratories.	3		1		3
8) It has the knowledge to register and accept all histopathological and cytopathological samples coming to the Pathology Laboratory.
9) It prepares fixatives of all tissue and cytological samples and ensures their fixation.	3
10) The performs the macroscopic sampling and identification of histopathological specimens at a basic level, with an expert pathologist.
11) It processor various tissue samples in a manual and/or automatic processor system.	3
12) According to the anatomical features of the tissues coming out of the tissue processor; embedded in paraffin with correct coding and orientation.	3
13) It uses a microtome within the framework of safety rules, takes sections from the paraffin block with the desired properties and thickness.	3
14) Prepares all dyes and auxiliary solutions used in the routine Hematoxylin-Eosin staining system, applies manual staining to all histopathological sections, checks staining under the microscope, detects the source of errors and solves the problem.	3
15) It provides a safe working environment in the intraoperative process, prepares preparations from biopsy samples and makes them ready for examination under the microscope.	2	3
16) Makes the appropriate choice between conventional histochemical staining methods to show various macromolecules of tissues.
17) Prepares the dye solutions to be used in conventional histochemistry methods and applies the methods, checks the staining under the microscope, determines the source of the error and solves the problem.	3		3
18) Immunohistochemistry applies FISH, SISH, CISH methods manually. Solves and records the problems that may occur as a result of the application, together with an independent or expert pathologist.				3
19) It provides fixation of conventional pap smear and liquid-based cervical cytology specimens. Starts and applies the painting process.				2
20) Makes macroscopic definitions of non-gynecological cytological samples, prepares preparations and performs fixation. Prepares and prepares the cell block. Starts and applies the painting process.	3	3
21) It isolates DNA from paraffin embedded tissue and other biological materials.
22) It performs the amplification of the optimized gene region by the polymerase chain reaction (PCR) reaction method and shows the amplification result by the gel electrophoresis method. Problems that may arise as a result of the application, independently or together with experts, solve the problem and record it.
23) Performs the use and maintenance of devices and equipment used in pathology laboratories and ensures their functionality.
24) Gains the skills of preparing various fixatives and fixation of all organs, performing tissue processor in a manual and/or automatic system, embedding tissue and sectioning in a microtome at desired thickness.	3
25) Have the ability to prepare and dye hematoxylin-eosin dye solutions.	3
26) Have the ability to prepare histochemistry dye solutions and apply various dyeing techniques.	3
27) Have the ability to apply Immunohistochemistry and Molecular Pathology Techniques.				3
28) Have the ability to perform cytopreparation and screening of gynecological and non-gynecological cytology samples.
29) Have the ability to identify the problems that may occur in the pathology laboratory in the light of scientific data/evidence and produce solutions.	3
30) Pathology Laboratory Techniques will have the ability to follow up-to-date methods and technologies related to professional practices.
31) Have the ability to follow the developing quality processes in the Pathology Laboratory and to include them in their professional life.	3
32) Acts in accordance with the laws, regulations, regulations and professional ethics rules regarding his duties, rights and responsibilities as an individual.
33) It gives information about the service carried out depending on the needs of the patient.
34) It organizes the work, takes personal responsibility when necessary, while fulfilling its professional duties with the awareness of teamwork.
35) He/she conveys his/her thoughts through written and verbal communication at the level of basic knowledge and skills on the subjects related to his/her field.
36) It performs the preparation of all tissue, gynecological and non-gynecological cytology samples, detects possible problems and produces solutions.	3	3
37) It makes a regular archive by providing long-term storage conditions of all tissue blocks and preparations in the pathology laboratory.
38) It uses conventional and advanced devices in the pathology laboratory with the principles of safe use, and maintains them in the light of current instructions.					3
39) Knows the necessary equipment and consumables for the functioning of the Pathology Laboratory, monitors the laboratory warehouse.
40) Have knowledge and skills on prevention and first aid from biological, physical and chemical hazards that may be encountered in the pathology laboratory.	3	1
41) Can apply basic first aid.

Course Teaching, Learning Methods

Q & A
Case Problem Solving/ Drama- Role/ Case Management
Laboratory
Quantitative Problem Solving
Fieldwork
Group Study / Assignment
Individual Assignment
WEB-based Learning
Internship
Practice in Field
Project Preparation
Report Writing
Seminar
Supervision
Social Activity
Occupational Activity
Occupational Trip
Application (Modelling, Design, Model, Simulation, Experiment et.)
Reading
Thesis Preparation
Field Study
Student Club and Council Activities
Other
Logbook
Interview and Oral Conversation
Research
Watching a movie
Bibliography preparation
Oral, inscribed and visual knowledge production
Taking photographs
Sketching
Mapping and marking
Reading maps
Copying textures
Creating a library of materials
Presentation

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance		% 0
Laboratory		% 0
Application		% 0
Practice Exam	1	% 30
Quizzes		% 0
Homework Assignments		% 0
Presentation		% 0
Project		% 0
Special Course Internship (Work Placement)		% 0
Field Study		% 0
Article Critical		% 0
Article Writing		% 0
Module Group Study		% 0
Brainstorming		% 0
Role Playing + Dramatizing		% 0
Out of Class Study		% 0
Preliminary Work, Reinforcement		% 0
Application Repetition etc.		% 0
Homework (reading, writing, watching movies, etc.)		% 0
Project Preparation + Presentation		% 0
Report Preparation + Presentation		% 0
Presentation / Seminar Preparation + Presenting		% 0
Oral examination		% 0
Midterms		% 0
Final	1	% 60
Report Submission	1	% 10
Bütünleme		% 0
Kanaat Notu		% 0
Committee		% 0
Yazma Ödev Dosyası		% 0
Portfolio		% 0
Take-Home Exam		% 0
Logbook		% 0
Discussion		% 0
Participation		% 0
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

Calculation of Workload and ECTS Credits

Activities	Number of Activities	Workload
Course Hours	14	112
Laboratory	13	78
Application
Practice Exam
Special Course Internship (Work Placement)
Field Work
Study Hours Out of Class
Article Critical
Article Writing
Module Group Study
Brainstorming	14	14
Role Playing + Dramatizing
Out-of-Class Study (Pre-study, Reinforcement, Practice Review, etc.)
Homework (reading, writing, watching movies, etc.)
Project Preparation + Presentation
Report Preparation + Presentation	14	14
Presentation / Seminar Preparation + Presenting
Oral examination
Preparing for Midterm Exams
MIDTERM EXAM (Visa)
Preparing for the General Exam
GENERAL EXAM (Final)
Participation
Discussion
Portfolio
Take-Home Exam
Logbook	14	14
Total Workload		232
ECTS (30 saat = 1 AKTS )		7