General Information about the Course

Course Code:	TLT207
Course Title:	Molecular Biological Methods
Course Semester:	3. Semester / Fall
Course Credits:	Theoretical Practical Credit ECTS 2 3 4 4
Language of instruction:	TR
Prerequisite of the course:	No
Ders İş Deneyimini Gerektiriyor mu?:	No
Type of course:	Necessary
Level of course:	Associate TR-NQF-HE:5. Master`s Degree QF-EHEA:Short Cycle EQF-LLL:5. Master`s Degree
Course Lecturer(s):	Öğretim Üyesi Nilay Yönet
Course Assistants:

Purpose and content of the course

Course Objectives:	Molecular biological methods are widely used in medical laboratories for diagnosis and patient monitoring. In this course, it is aimed for students to learn the main working principles of molecular biological methods used in medical laboratories.
Course Objective:	To learn the fundamentals of analysis of molecular biology techniques, to be able to apply these techniques under laboratory conditions, to evaluate the application results, to express and discuss them in writing, and to optimize the laboratory application.
Mode of Delivery:	Face to face

Learning Outcomes

Knowledge (Described as Theoritical and/or Factual Knowledge.)

1) By understanding the theoretical foundations and laboratory applications of molecular biological methods, students will gain the competence to plan, conduct and analyze complex experimental procedures in biotechnology and medical laboratory environments by using this knowledge. In this way, students will acquire the molecular biological skills and technical knowledge necessary to work professionally in the biotechnology sector and research laboratories.

Skills (Describe as Cognitive and/or Practical Skills.)

1) Students will understand the theoretical foundations of molecular biological methods and, using this knowledge, will be able to successfully apply basic molecular biology techniques such as DNA isolation, PCR, gel electrophoresis and sequencing in the laboratory environment. Additionally, they will gain competence in developing and testing hypotheses in biological research by interpreting the data obtained. This course outcome aims to combine students' theoretical knowledge in the field of molecular biology with practical applications. During the course, students will learn the basic techniques of molecular biology and gain experience by applying these techniques in laboratory studies. They will also develop critical thinking and problem-solving skills in biological research by analyzing and interpreting laboratory results. This achievement enables students to build both theoretical and practical knowledge in the field of molecular biology.

Competences (Described as "Ability of the learner to apply knowledge and skills autonomously with responsibility", "Learning to learn"," Communication and social" and "Field specific" competences.)

1) Students will gain the ability to work independently on molecular biological techniques and methods and will take responsibility for laboratory projects, taking an active role in planning, executing experiments and reporting the results. In this process, they will improve their time management, resource use and result-oriented working skills. This course outcome aims to improve students' ability to work independently and take responsibility in molecular biology laboratories. During the course, students will plan and implement their projects individually, analyze and report the experimental results. These experiences will increase students' competencies in working independently, solving problems and taking scientific responsibility. Thus, they will be able to effectively take part in laboratory projects and assume responsibilities in their professional life after graduation.

2) Students will acquire the competency of continuous learning and self-improvement in the field of molecular biological methods; specifically, they will develop the ability to follow scientific literature, learn new techniques and technologies, update their existing knowledge, and independently acquire new skills and knowledge. This course outcome aims to enhance students' abilities for continuous learning and self-improvement in molecular biology. Students will gain the competency to follow scientific articles and current literature, learn about new developments, and keep their existing knowledge up to date. Throughout this process, they will reinforce their independent learning skills, enabling them to continuously improve in their scientific research and professional careers.

3) Students will acquire advanced technical knowledge and skills in the field of molecular biological methods; specifically, they will be able to perform fundamental molecular biology techniques such as DNA/RNA isolation, PCR, gel electrophoresis, and genetic analyses, and apply these techniques in professional settings such as biotechnology and medical laboratories. This course outcome aims to equip students with professional-level knowledge and skills in molecular biology. Throughout the course, students will learn and apply advanced molecular biology techniques and develop the competency to effectively use these techniques in professional laboratory environments. As a result, they will be prepared to successfully undertake specialized tasks in fields such as biotechnology and medical laboratories upon graduation.

Course Topics

Week	Subject
Related Preparation	Pekiştirme
1)	Biosafety
2)	General structure of DNA and RNA
3)	Codon-anticodon system, reading the genetic code
4)	DNA examination methods
5)	DNA isolation and application
6)	Polymerase Chain Reaction
7)	Extracting genomic DNA from blood
8)	Midterm
9)	Preparation and application of agarose gel
10)	DNA Imaging, reading and evaluation
11)	Cell and Tissue Culture
12)	Southern, Northern, Western Blotting Methods
13)	DNA cloning and Genetic Engineering Methods
14)	DNA Sequencing Methods
15)	Final Exam
References: 1. Molecular Biology Techniques: An Intensive Laboratory Course (Walt Ream, Katharine G. Field), 1999. 2. Moleküler Biyolojide Kullanılan Yöntemler, Prof.Dr. Güler Temizkan, Prof.Dr.Nazlı Arda, Nobel Tıp Kitabevleri, 2008. 3. Moleküler Biyolojide Kullanılan Yöntemler-Temizkan,Arda-Nobel Tıp Kitabevi 2004 4. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2014). Molecular Biology of the Cell (6th ed.). Garland Science. 5. Cox, M. M., Doudna, J. A., & O'Donnell, M. (2015). Molecular Biology: Principles and Practice (2nd ed.). W.H. Freeman and Company.

Ders - Program Öğrenme Kazanım İlişkisi

No Effect	1 Lowest	2 Average	3 Highest

Ders Öğrenme Kazanımları	1	2	3	4	5
Program Outcomes
1) To have basic information about the normal structure-functions of human systems, to analyze this information at the tissue-cell level and to associate it with diseases.	3	2	2	2	2
2) To have knowledge about the work organization in medical laboratories and to be able to carry out applications.	2	3	2	1	2
3) To be able to take appropriate samples from patients for medical laboratory tests and to transfer samples under appropriate conditions.	1	1	3
4) To be able to do sample preparation before analysis, to prepare maintenance preparations, to apply disinfection and sterilization techniques.	1	1	1	3	1
5) To be able to apply basic tests related to different areas of medical laboratory, to prepare analysis solutions.	3	3	3	3	3
6) To be able to associate medical laboratory tests with metabolism and structure-functions of body systems or microbial/parasitic factors.	1	1	1	2	2
7) To be able to reveal the sources of error in the total testing process in medical laboratories and to produce solutions.	1	1	2	2	2
8) To be able to use information technologies in medical laboratory applications and to improve themselves by using information technologies.
9) To be able to define medical laboratories quality management systems, to carry out quality control processes, to use statistical methods in these applications.	1	1	1	1	1
10) To be able to apply safety rules in medical laboratories, to take personal safety precautions and to create a safe laboratory environment.	1	1	2	2	3
11) To be able to apply environmental health and waste management measures in medical laboratory studies, to have knowledge about environmental protection legislation.	1	1	2	2	2
12) Who has sufficient knowledge about the devices and chemicals in the working environment and undertakes the tasks assigned to that one. In extraordinary situations that may disrupt the workflow, it primarily contributes to solving problems individually and, if necessary, as a team.
13) Be open to scientific and technological developments and follow them.	1	1	1	1	1
14) Evaluates the basic level of knowledge and skills acquired in the field with a critical approach; identifies learning needs and obtains gains.
15) Ability to communicate individually and socially, and work as a team in the laboratory and in the institution where the laboratory is located.	1	1	1	2	2
16) Can communicate effectively in oral and written form and uses Turkish effectively in the execution of health services.
17) Being able to speak and write English at an intermediate level, use a foreign language in professional practice.
18) It can apply first aid in emergency situations, provide basic life support, and transport the sick and injured.
19) Has knowledge about defining general ethical and moral concepts, complying with the principles of professional and medical ethics, special medical ethical issues and patient rights.

Course Teaching, Learning Methods

Q & A
Case Problem Solving/ Drama- Role/ Case Management
Laboratory
Quantitative Problem Solving
Fieldwork
Group Study / Assignment
Individual Assignment
WEB-based Learning
Internship
Practice in Field
Project Preparation
Report Writing
Seminar
Supervision
Social Activity
Occupational Activity
Occupational Trip
Application (Modelling, Design, Model, Simulation, Experiment et.)
Reading
Thesis Preparation
Field Study
Student Club and Council Activities
Other
Logbook
Interview and Oral Conversation
Research
Watching a movie
Bibliography preparation
Oral, inscribed and visual knowledge production
Taking photographs
Sketching
Mapping and marking
Reading maps
Copying textures
Creating a library of materials
Presentation

Evaluation System

Semester Requirements	Number of Activities	Level of Contribution
Attendance		% 0
Laboratory	14	% 20
Application		% 0
Practice Exam		% 0
Quizzes		% 0
Homework Assignments		% 0
Presentation		% 0
Project		% 0
Special Course Internship (Work Placement)		% 0
Field Study		% 0
Article Critical		% 0
Article Writing		% 0
Module Group Study		% 0
Brainstorming		% 0
Role Playing + Dramatizing		% 0
Out of Class Study		% 0
Preliminary Work, Reinforcement		% 0
Application Repetition etc.		% 0
Homework (reading, writing, watching movies, etc.)		% 0
Project Preparation + Presentation		% 0
Report Preparation + Presentation		% 0
Presentation / Seminar Preparation + Presenting		% 0
Oral examination		% 0
Midterms	1	% 20
Final	1	% 60
Practical Final		% 0
Report Submission		% 0
Bütünleme		% 0
Bütünleme Pratik		% 0
Kanaat Notu		% 0
Committee		% 0
Yazma Ödev Dosyası		% 0
Portfolio		% 0
Take-Home Exam		% 0
Logbook		% 0
Participation		% 0
Discussion		% 0
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

Calculation of Workload and ECTS Credits

Activities	Number of Activities	Workload
Course Hours	14	28
Laboratory	14	42
Application
Practice Exam
Special Course Internship (Work Placement)
Field Work
Study Hours Out of Class	14	14
Article Critical
Article Writing
Module Group Study
Brainstorming
Role Playing + Dramatizing
Out-of-Class Study (Pre-study, Reinforcement, Practice Review, etc.)
Homework (reading, writing, watching movies, etc.)	14	14
Project Preparation + Presentation
Report Preparation + Presentation
Presentation / Seminar Preparation + Presenting
Oral examination
Preparing for Midterm Exams
MIDTERM EXAM (Visa)	1	5
Preparing for the General Exam
GENERAL EXAM (Final)	1	5
Participation	14	14
Discussion
Portfolio
Take-Home Exam
Logbook
Total Workload		122
ECTS (30 saat = 1 AKTS )		4